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HCG is produced by the syncytiotrophoblast of the placenta and can be detected in maternal serum immediately upon nidation. HCG is responsible for maintain the function of the ovarian corpus luteum. In normal pregnancy, HCG concentrations rapidly increase with a doubling rate of approximately two days. Pathologically decreased values indicate abnormal pregnancy (e.g. ectopic pregnancy). A sudden fall of HCG levels before the 8th -10th week of gestation should be considered as a serious indicator of imminent abortion. If pregnancy can be excluded, HCG levels above normal are highly likely to indicate the presence of malignant neoplasms, particularly germ-cell tumors (e.g. testicular tumors, chorionic or ovarian carcinoma. For use as a tumor marker, the test system has to recognize both HCG and β-HCG, since testicular and chorionic carcinomas mainly secrete the free β-chains rather than the intact HCG molecule\nPRINCIPLE OF THE TEST Sandwich immunoluminometric assay: Use an anti-free-β-HCG monoclonal antibody to label ABEI, and use another monoclonal antibody to label FITC. Sample, Calibrators, or Control with ABEI Label, FITC Label and magnetic microbeads coated with anti-FITC are mixed thoroughly and incubated at 37℃, forming a sandwich; After sediment in a magnetic field, decant the supernatant, cycle washing for 1 time. Subsequently, the starter reagents are added and a flash chemiluminescent reaction is initiated. The light signal is measured by a photomultiplier as RLU within 3 seconds and is proportional to the concentration of free-β-HCG present in samples. 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